Mass Spectrometry and Proteomics training : Monday, November 23th - Friday, November 27th 2020

GOALS

Acquiring the theoretical bases and minimum practical requirements to interpret mass spectra obtained form micro proteinic quantities.Retour ligne automatique
Initiation to the identification of proteins by searching sequence databases.Retour ligne automatique
PUBLIC

Superior technicians, engineers, researchers.

PROGRAM

Courses

Macrobiomolecular ionization techniques of biological interest : ESI, MALDI.Retour ligne automatique
Analyzers : TOF, quadrupoles, ion traps, hybrid analyzers, FT-ICR, TOF/TOF.Retour ligne automatique
Molecular mass measurement (MS),Retour ligne automatique
Peptides, fragmentation rules, spectra interpretation, computer assisted interpretation, (MS/MS).Retour ligne automatique
Sample preparation, digestion.Retour ligne automatique
Identification of proteins by Mass Finger printing.Retour ligne automatique
Identification by partial sequencing in MS/MS.Retour ligne automatique
IT tools used to search databases.Retour ligne automatique
HPLC and nano HPLC coupling.Retour ligne automatique
Introduction to the differential quantification by MS.Retour ligne automatique
Practical skills :

Identification of proteins by mass spectrometry : from the protein band isolated in an acrylamid gel to its identification.

You can bring your own samples if you wish. Contact us for more practical details.
To register : https://cnrsformation.cnrs.fr/stage-20156-Caracterisation-des-proteines-par-spectrometrie-de-masse-dans-le-contexte-de-la-proteomique.html?mc=proteine