Opening of a 18 months postdoctoral position in January 2025

Position affiliation

The postdoctoral researcher will be affiliated with the Laboratory of Biological Mass Spectrometry
and Proteomics at ESPCI Paris (SMBP, led by Joelle Vinh), under the supervision of Giovanni
Chiappetta. The project is in collaboration with Agnes Delaunay-Moisan’s group at CEA Saclay.

Presentation of the project :

The ERXCUE project, funded by the ANR, aims to study how cells adapt—or fail to adapt—to
endoplasmic reticulum (ER) stress, with a focus on the remodeling of thiol-redox metabolism. The
goal is to identify the redox processes that influence the cellular decision between survival and
apoptosis during the unfolded protein response (UPR). By combining unbiased -omics approaches
with targeted studies at the subcellular proteomic level, the postdoctoral researcher will explore
how redox activities contribute to the regulation of the UPR. The project is part of a collaboration
with Agnes Delaunay-Moisan’s group at CEA Saclay.

Responsibilities :

• Proteomics and Redoxomics Analysis :
o Design and conduct mass spectrometry experiments (LC-MS/MS) to identify and
quantify cysteine redox post-translational modifications in proteins involved in the UPR.
o Focus on thiol modifications and redox-active proteins.
• Data Integration :
o Integrate redox proteomics data with transcriptomics data to create a comprehensive
map of redox pathways regulated by the UPR.
o Use bioinformatics tools to correlate proteomic data with cellular redox states and UPR
transitions.
• Experimental Design :
o Develop and optimize methods for subcellular fractionation and redoxomics targeting
specific organelles, particularly the endoplasmic reticulum (ER).
o Implement innovative workflows to map ER-specific redox modifications and redox
activity under UPR stress conditions.
• Collaborative Research :
o Work closely with redox biology and metabolomics teams to understand how changes in
thiol-redox metabolism and other pathways affect cellular responses to ER stress.
o Study metabolic remodeling under UPR and its impact on the proteome.

Profile

Desired knowledge and skills :
• Principles and experience in proteomics analysis
• Handling quantitative proteomics data
• Human cell culture
• Knowledge of R programming language (preferred, not mandatory)

Degree :

PhD in Biochemistry, Analytical Chemistry, or Biology
Preferred experience :
Early-career researcher (1st postdoc)
Position available from :
January 2025.

Contact

Applications (CV, cover letter) should be submitted by email to

Address
10, Rue Vauquelin 75005 Paris
Métro 7 (Place Monge/Censier Daubenton) - RER B (Luxembourg) - Bus 21, 27 & 47